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Biochemical Society Transactions. 2000; 28: 4.

Spinal fusion with rhGDF-5/MP52 in combination with collagen matrix.

By: Spiro RC, Liu LS, Heidaran MA, Thompson AY, Ng CK, Pohl J, Poser JW.

Growth and differentiation factor-5 (GDF-5) is a divergent member of the transforming growth factor-ß/bone morphogenetic protein (BMP) superfamily that is required for proper skeletal patterning and development in the vertebrate limb.
Based on the homology of GDF-5 with other bone-inducing BMP family members, the inductive activity of a recombinant form of human GDF-5 (rhGDF-5/MP52) was evaluated in a series of in vitro assays and in vivo bone-formation models.
The in vitro response to rhGDF-5/MP52 resulted in the formation of chondrogenic nodules in fetal rat calvarial cells cultured in the context of collagen or collagen/hyaluronate extracellular matrices.Matrices loaded with rhGDF-5 induced ectopic cartilaginous and osseous tissue when implanted in subcutaneous or intramuscular sites.
In non-human primate long-bone-defect and spinal-fusion models, rhGDF-5/MP52 combined with a mineralized collagen matrix induced bone formation in a manner equivalent to autogenous bone.
These results highlight the unique potential of rhGDF-5/MP52 in a wide variety of orthopaedic applications.


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